Detecting Rare Thrombophilia Variants by High-Resolution Melting
نویسندگان
چکیده
منابع مشابه
Detecting rare thrombophilia variants by high-resolution melting.
Genotyping by amplicon melting is a minimalistic approach to geno-typing, requiring only PCR, a fluorescent DNA dye, and single-color acquisition of a melting curve in a closed tube. Multiplex genotyping for the F5 1 [coagulation factor V (proaccelerin, labile factor)] Le-iden (1691GϾA), F2 [coagulation factor II (thrombin)] 20210GϾA, and MTHFR [methylenetetrahy-drofolate reductase (NAD(P)H)] 1...
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BACKGROUND Heteroduplex scanning techniques usually detect all heterozygotes, including common variants not of clinical interest. METHODS We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen I, and all 768 ...
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Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detecti...
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High-resolution amplicon melting is a simple method for genotyping that uses only generic PCR primers and a saturating DNA dye. Multiplex amplicon genotyping has previously been reported in a single color, but two instruments were required: a carousel-based rapid cycler and a high-resolution melting instrument for capillaries. Manual transfer of capillaries between instruments and sequential me...
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Genetic variability is a key problem in the prevention and therapy of RNA-based virus infections. Infectious Salmon Anemia virus (ISAv) is an RNA virus which aggressively attacks salmon producing farms worldwide and in particular in Chile. Just as with most of the Orthomyxovirus, ISAv displays high variability in its genome which is reflected by a wider infection potential, thus hampering manag...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2011
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2010.159160